Bioactive compounds from freshwater crab (Potamon ebonyicum) was extracted and characterized in Ebonyi River Basin. Chitosan was extracted by standard methods which involved demineralization, deproteinization and deacetylation of chitin from male and female crabs. Bioactives from the chitosan (polysaccharide, peptide, alkaloid, and lipid) were quantified and analysed using one way analysis of variance (ANOVA). The result revealed sex based variation in bioavailability and accumulation of the bioactive compounds. The female crab recorded more chitosan yield, and significantly higher (p < 0.05) polysaccharide than the male crab. The male has higher concentration of peptide, alkaloid and lipid than the female crab. Subsequently and in terms of production of polysaccharide, the female P. ebonyicum should be recommended while the male could be used for the production of peptide, alkaloid and lipid. The findings might strategically support the development of local extraction and utilization of bioactive compounds for positive impacts on pharmaceutical industries and the economy.