ARCHIVES
VOL. 8, ISSUE 2 (2026)
Effects of different concentrations of growth regulators on the in vitro callusogenesis of Lavandula angustifolia Mill
Authors
Ramune Masiene, Rita Asakaviciute, Natalija Burbulis
Abstract
Lavandula angustifolia Mill. is an important
ornamental and medicinal plant, widely valued for its essential oil and
horticultural applications. Efficient in vitro propagation requires the
optimisation of explant type and plant growth regulator combinations to ensure
reliable callus induction. This study aimed to evaluate the callogenic
potential of leaf and stem explants of L. angustifolia under different
auxin–cytokinin regimes. Explants were cultured on Murashige and Skoog medium
supplemented with various concentrations and combinations of
6-benzylaminopurine (BAP), α-naphthaleneacetic acid (NAA), and kinetin. Callus
induction frequency was assessed after four weeks of cultivation. Leaf explants
exhibited a higher callus induction frequency, reaching up to 100%, indicating
superior regenerative responsiveness, whereas stem explants produced a
significantly greater callus biomass. The most effective hormonal combination
for callus formation was 1.5 mg l⁻¹ BAP combined with 1.0 mg l⁻¹ NAA. The
results demonstrate that both explant type and growth regulator composition
critically influence callusogenesis in L. angustifolia, providing a
practical basis for improving in vitro propagation protocols for
horticultural production and further biotechnological applications.
Download
Pages:67-71
How to cite this article:
Ramune Masiene, Rita Asakaviciute, Natalija Burbulis "Effects of different concentrations of growth regulators on the <i>in vitro </i>callusogenesis of <i>Lavandula angustifolia</i> Mill". International Journal of Biotechnology and Microbiology, Vol 8, Issue 2, 2026, Pages 67-71
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.