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VOL. 8, ISSUE 1 (2026)
Antioxidative and enzymatic profiling of radish (Raphanus sativus) seeds under salinity stress
Authors
Kritika Sonkusale, Shivali Tiwari, Dr. Anju Meshram
Abstract
Salinity stress is a major abiotic factor that adversely affects seed
germination and plant development, particularly by inducing oxidative stress
through excessive production of reactive oxygen species (ROS). This study
investigates the enzymatic antioxidant response in Raphanus sativus
(radish) seeds subjected to varying concentrations of saline solutions (NaCl).
Seeds were germinated under controlled laboratory conditions with incremental
salinity levels (0, 10, 50 and 100 mM NaCl) and the activity of key antioxidant
enzymes— catalase (CAT) and Ascorbate peroxidase (APX) as well as protein
content was quantified. The results revealed a concentration-dependent
modulation in enzyme activity, with moderate salinity (up to 10-50 mM)
triggering enhanced antioxidant defence mechanisms, while higher concentrations
(50–100 mM) led to reduced enzymatic activity, indicating possible oxidative
damage. This enzymatic profiling underscores the role of antioxidative enzymes
in mitigating salinity-induced stress during seed germination and suggests a
threshold of salt tolerance in Raphanus sativus seeds. The findings
contribute to understanding the biochemical resilience of radish under salt
stress, providing a basis for breeding or engineering salt-tolerant cultivars.
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Pages:88-95
How to cite this article:
Kritika Sonkusale, Shivali Tiwari, Dr. Anju Meshram "Antioxidative and enzymatic profiling of radish (<i>Raphanus sativus</i>) seeds under salinity stress". International Journal of Biotechnology and Microbiology, Vol 8, Issue 1, 2026, Pages 88-95
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