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VOL. 7, ISSUE 4 (2025)
In vitro seed germination, differentiation and PLBs multiplication of the medicinal orchid Pholidota imbricata Hook. F. from Bangladesh
Authors
Awishik Tripura, Marzia Akter Sumi, Minhajur Rahman, Tapash Kumar Bhowmik
Abstract
Pholidota imbricata
Hook. f. is an epiphytic medicinal orchid native to Bangladesh, valuable for
its therapeutic applications in treating rheumatic pain and bone fractures. Due
to habitat destruction and the inherent difficulty of natural seed germination
caused by the lack of endosperm, this species faces conservation threats. This
study established a reliable in vitro propagation protocol via
asymbiotic seed germination and Protocorm-Like Bodies (PLBs) multiplication.
Four basal media viz. Murashige and Skoog (MS), Phytamax (PM), Modified
Vacin and Went (MVW) and Knudson C (KC) were evaluated. The highest seed
germination rate (93.34%) and earliest protocorm development (8.13 weeks) were
achieved on MS medium supplemented with 0.5 mg/l BAP and 0.5 mg/l NAA. For mass
multiplication, PLBs were cultured on MS medium fortified with various
concentrations of 2,4-D and BAP. The synergistic combination of 2.0 mg/l 2,4-D
and 0.8 mg/l BAP proved most effective, yielding the highest fresh weight (1.53
g) and number of PLBs (88.2) per vessel after 60 days of inoculation.
Regenerated plantlets were successfully acclimatized with a 65% survival rate.
This protocol provides a viable pathway for the mass propagation and ex situ
conservation of this important medicinal orchid.
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Pages:77-80
How to cite this article:
Awishik Tripura, Marzia Akter Sumi, Minhajur Rahman, Tapash Kumar Bhowmik "<i>In vitro</i> seed germination, differentiation and PLBs multiplication of the medicinal orchid <i>Pholidota imbricata</i> Hook. F. from Bangladesh". International Journal of Biotechnology and Microbiology, Vol 7, Issue 4, 2025, Pages 77-80
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