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VOL. 7, ISSUE 4 (2025)
Isolation and Characterization of Microorganisms from Chicken Droppings
Authors
Onyeulor, P N, Okore C N, Okpe C J, Arimanwa I C
Abstract
Humans have continued to research into the
role of organic manure as alternative to inorganic manure. Poultry droppings
have served as alternative source of nitrogen, phosphorous and potassium to
crops. It is therefore important to investigate into the possibility of these
droppings to serve as vector to zoonotic pathogens which may be detrimental to
the health of the farmer, if not well managed. Isolation and characterization
of microorganisms from some poultry houses were carried out. Poultry droppings
were collected from poultry owners around Federal Polytechnic Nekede, Owerri,
Imo State. About 100g of the sample was collected into a sterile container
using sterile spatula and immediately taken to the laboratory in cold chain for
analysis. Different media were used for the analysis and they were prepared
based on manufacturers’ specification. Tenfold serial dilution of the samples
was made and spread plate technique was used in the isolation. Both
morphological and biochemical characteristics were used in the identification
of the isolates. The results obtained showed that microbial load of chicken
droppings ranged from 1.0 x 103 to 2.0 x 105cfu/ml. Four
bacteria genera- Staphylococcus, Escherichia coli Bacillus and
Salmonella and four fungal genera - Penicillium, Rhizopus, Alternaria and
Sporotricum were identified to be present on the chicken droppings. It can
be concluded therefore that chicken droppings are potential vector of zoonotic
pathogens hence should be handled with care when being evacuated and used as
organic manure.
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Pages:45-48
How to cite this article:
Onyeulor, P N, Okore C N, Okpe C J, Arimanwa I C "Isolation and Characterization of Microorganisms from Chicken Droppings". International Journal of Biotechnology and Microbiology, Vol 7, Issue 4, 2025, Pages 45-48
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