Acinetobacter baumannii is a Gram-negative bacterium that is responsible for causing infection at different site the bacteria have been reported to induce infection in the urinary tracts, wound and burns areas specially in hospitalized cases. The current study embraced collecting specimen from 240 clinical cases to identify A. baumanni causes of infection through the period between February 2025 until June 2025 at Tikrit Teaching Hospital. All specimens underwent routine culture and biochemical identification in addition to confirmation via DL microbiology identification kit, Efflux activity was measured via Ethidium bromide method in presence and absence of Efflux inhibitor, in addition, DNA extraction was performed on all isolates to detect the presence of adeA gene. Our results revealed that males to female’s ratio was 40:60 while the mean age of them were 47 and 35 years respectively. A. baumanni was positive in