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VOL. 7, ISSUE 2 (2025)
Separation, purification and isolation of polymyxin b sulphate
Authors
Ravindra Krishnarao Burde, Dr. Amitabh Chaturvedi, Karunasagar KM, Mukesh Kumar, Dr. Umesh Luthra
Abstract
This study explores the purification of
Polymyxin B sulphate through resin and elution solvent optimization. Diaion
WK40L was found to be the most suitable for adsorption, impurity removal,
and simple desorption. It had an 85% output,
60.00% total batch yield, and 92.92% content of polymyxin B.
Ethanol was the best solvent for the Sephadex SP700 Resin
column, with a pH 3.0 solution of ethanol resulting in 98.68% resin stage
yeild, 60.00% overall yield, and 92.92% polymyxin B content. The pirification
was pharmaceutical compliant, eliminating toxic polymyxins B1-I and
B3, and surpassing the microbial assay requirement (9157.58
u/mg). The established method proves to be industrially applicable for attaining high
yield and pharmacopeial compliance, Polymyxin B Sulphate.
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Pages:44-48
How to cite this article:
Ravindra Krishnarao Burde, Dr. Amitabh Chaturvedi, Karunasagar KM, Mukesh Kumar, Dr. Umesh Luthra "Separation, purification and isolation of polymyxin b sulphate". International Journal of Biotechnology and Microbiology, Vol 7, Issue 2, 2025, Pages 44-48
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