Background: Patients with respiratory like illness were considered for the study and their viral load/severity of infection and Ct (Cycle threshold) values were compared based on number and severity of clinical symptoms.

Aim & Objective: To compare the severity of infection with Ct value in both viral and bacterial-viral co-infections.

Methods and Material: Swab samples were taken and subjected to RNA Extraction along with RT-PCR using standard protocol, to determine positive and negative samples and to find out Ct values of positive cases for further analysis.

Result: Influenza A positive subjects showed more severe respiratory symptoms in comparison to the negative cases. And among positive cases, a clear trend is seen in which individuals with lower Ct values, indicative of higher viral loads exhibited more severe respiratory symptoms and patients with higher Ct values showed milder clinical presentations despite having multiple comorbidities.