Biofilms are complex microbial communities encased in a self-produced extracellular matrix, which contribute significantly to bacterial persistence and resistance to antimicrobial agents. Pseudomonas aeruginosa and Staphylococcus aureus are among the most common biofilm-forming pathogens, often implicated in chronic infections and medical device-related complications. This study investigates the efficacy of sodium hypochlorite in disrupting and removing biofilms formed by these bacteria. Using quantitative analysis and microscopy techniques, we assessed the impact of different concentrations and exposure times of sodium hypochlorite on biofilm degradation. The results indicate that sodium hypochlorite effectively reduces biofilm biomass and disrupts bacterial viability, with higher concentrations showing a more pronounced effect. These findings underscore the potential of sodium hypochlorite as an effective biofilm control agent in healthcare and industrial settings.