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VOL. 7, ISSUE 1 (2025)
Quantitative determination of unconjugated bilirubin in serum of human
Authors
Farah S Daabool, Faiq F Karam
Abstract

Unconjugated bilirubin (UCB) is a powerful antioxidant and cytoprotectant, but at pathologically high amounts, it also induces apoptosis and cytotoxicity. To assess the function of UCB concentrations in redox control, Accurate measures of UCB concentrations in cells, fluids, and tissues are necessary for the prevention of bilirubin encephalopathy, atherosclerotic diseases, and cancer. In the present study, we evaluated a sensitive method for tissue UCB calculation.

Losses of UCB after extraction were compensated for by the use of mesobilirubin as an internal standard. Recovery rates tended to be 75%. The cutoff for detection was 10 pmol UCB/g of moist tissue. There was a 2.5% variance. When utilized to evaluate UCB concentrations in human tissues, this method yielded UCB values that were directly comparable to current methods and correctly recognized very low tissue bilirubin concentrations (40 pmol UCB/g tissue) in non-jaundiced.
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Pages:27-29
How to cite this article:
Farah S Daabool, Faiq F Karam "Quantitative determination of unconjugated bilirubin in serum of human". International Journal of Biotechnology and Microbiology, Vol 7, Issue 1, 2025, Pages 27-29
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