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VOL. 6, ISSUE 4 (2024)
Bacterialogical examination of well water samples to confirm faecal contamination
Authors
Aneke Jacinta Chinwe
Abstract
A bacteriological analysis was conducted on well
water samples in Enugu Metropolis to address the rising incidence of
waterborne diseases. The study employed the Most Probable Number (MPN)
technique at 37°C, utilizing MacConkey broth and Eosine methylene blue, to
assess the microbial quality of the well water. Standard methods, including
presumptive, confirmatory, and completed tests, were performed to detect Escherichia
coli. The isolated E.coli was further characterized through gram
staining and biochemical tests, and the Eijkman method was used to confirm its
faecal origin. Wells with covered and unplastered interiors, particularly those
near septic tanks and gutters, showed higher levels of faecal contamination. In
areas like Abakpa, Maryland, Achara Layout, and Emene, well water is
microbiologically unsafe for consumption or domestic use, requiring additional
treatment measures such as boiling or disinfection. Proximity to pollution
sources poses a significant risk of waterborne disease outbreaks, emphasizing
the need for prevention and adherence to World Health Organization standards
for untreated water.
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Pages:6-10
How to cite this article:
Aneke Jacinta Chinwe "Bacterialogical examination of well water samples to confirm faecal contamination". International Journal of Biotechnology and Microbiology, Vol 6, Issue 4, 2024, Pages 6-10
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