Problem: Mycotic keratitis is commonly considered as a resistant ulcer and a main reason for vision impairment. Its treatment was long time challenge with commercial drugs and low safety of new trials.

Aim: the present work will search for antifungal activity of a natural extract while considering the safety and minimal side effects on the treated corneal tissues.

Methods: The most common causative agent was isolated and identified from human corneal ulcers. Aqueous extract was prepared for Commiphora molmol, its MIC was determined against the tested isolate, comparing with the commercial antifungal agent. GC-MS analysis investigated the active ingredients of the selected plant extract. Its antimicrobial activity and histological effects were investigated on rabbit eyes.

Results: Candida albicans was found to be the most common yeast corneal ulcer invader. It was greatly inhibited by the aqueous myrrh extract with MIC of 200 µg/ml. that can be supported by normal appearance and minimal side effects on the treated corneal tissues in comparison with fluconazole as a commercial agent. These combined antimicrobial and anti-inflammatory actions referred to the presence of 2-Pentanone, 4-hydroxy-4-methyl; Undecanal; Indan-1,3-diol monopropionate; Triacetin and other plant components.