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VOL. 6, ISSUE 3 (2024)
The nematicidal effect of aqueous leaf extract of Crotolaria juncea l. in the control of meloidogyne species infecting two varieties of Solanum tuberosum l. (potato) in Jos, Nigeria
Authors
Folorunsho D A, Danahap L S, Nnebechukwu I A, Utoblo O G and Okechalu O B
Abstract
The presence of bioactive substances in plant extract with nematicidal
activity on Meloidogyne spp. to reduce crop damage, minimize chemical pesticide
toxicity to man and safety of the environment. Solanum tuberosum L.
(Potato) is one of the world’s most valuable and widely grown food crops,
because of its nutritional values, high yield and is ranked sixth with cereals.
The study investigated the nematicidal efficacy of aqueous leaf extract of Crotalaria
juncea L. in the control of Meloidogyne species infecting potato
varieties. The nematodes were isolated by modified funnel Baerman method and
identified by comparative morphology, descriptions and lattice keys. The
nematode suspension was prepared by taken 4 ml of the homogenized suspension
(108 juveniles) was subjected to the leaf extract at varied concentrations of
(0.75, 1.5, 3.0, 6.0 mg/mls). The set up was replicated three times and observed
daily for live and immobilized nematodes for four days. The setup was arranged
in a complete randomized design with five replicates and the two potato
varieties (Caruso and Marabel) roots were inoculated with second stage juvenile
root-knot nematodes at 2 weeks after planting (2WAP). The pots were drenched
with the leaf extract solutions at concentrations of (1.5, 3.0, 6.0 mg/mills)
at 4 weeks after planting (4WAP). Three controls (Furadan, inoculated +
unamended, un-inoculated + unamended) were used. The phytochemical analysis
indicated the presence of alkaloids, saponins, tannins, flavonoids, steroids
and cardiac glycosides. The results revealed that the higher the concentration
of the extract, the higher the mortality of root-knot nematodes. For instance,
6.0 mg/mls resulted in (100±00a) mortality by extract of C. juncea.
The in vivo results showed that at (p≤0.05) level of significance,
highest tuber weight was obtained from the plants treated with extract of C.
juncea. The test plant extract can be used to control root-knot nematodes
at a concentration of 6 mg/ml which had the highest mortality effect on the
nematodes, which suggested that it had potential of being formulated into a
biological nematicide.
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Pages:1-6
How to cite this article:
Folorunsho D A, Danahap L S, Nnebechukwu I A, Utoblo O G and Okechalu O B "The nematicidal effect of aqueous leaf extract of <i>Crotolaria juncea l. </i>in the control of <i>meloidogyne species </i>infecting two varieties of <i>Solanum tuberosum l</i>. (potato) in Jos, Nigeria". International Journal of Biotechnology and Microbiology, Vol 6, Issue 3, 2024, Pages 1-6
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