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VOL. 6, ISSUE 2 (2024)
Study the diversity of Nif H genes in the communities of nitrogen fixing bacteria in soil
Authors
Ashok Kr Singh
Abstract
The specific primers for the Nif H genes were tested with different
pure nitrogen fixing strains. A PolF/Polk primer set provided successful
amplification and the restriction enzyme NdelIl chosen for restriction fragment
length polymorphism (RFLP) analyses. These were the most discriminating for the
study of Nif H genes diversity as they resulted in differences between strains
at the species level. The amplification by selected primers and RFLP were
applied to assess the genetic diversity of the Nif H genes pool in soil. The
soils one under cultivation and other under permanent pasture were found to
harbor a contrasting diversity of Nif H genes. Pure strain profiles could not
be recognized in the Nif H genes soil patterns. This was shown that the
structure of nitrogen fixers in soil was influenced by soil functioning.
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Pages:33-35
How to cite this article:
Ashok Kr Singh "Study the diversity of Nif<i> </i>H genes in the communities of nitrogen fixing bacteria in soil". International Journal of Biotechnology and Microbiology, Vol 6, Issue 2, 2024, Pages 33-35
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