Recent research end eavors have focused on exploring hepatotoxicity resulting from the administration of certain pharmaceutical drugs and antibiotics, which elicit oxidative stress and elevate the production of free radicals. This perturbation leads to the disruption or inhibition of enzymatic and non-enzymatic antioxidant defense mechanisms within the liver. Following a two-month experimental period, the study subjects, i.e., animals, were subjected to anesthesia, and blood specimens were obtained via the ocular sockets for the purpose of assessing various biochemical parameters.

The biochemical analyses encompassed the determination of liver enzyme activities, specifically, alkaline phosphatase (ALP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT). The results derived from comprehensive statistical analysis unequivocally revealed the deleterious impacts of Mirabegron, characterized by a significant elevation in the levels of liver enzymes ALT, AST, and ALP. Notably, this effect was notably prominent at a concentration of 10 mg/kg. However, it is worth noting that ALP displayed a distinct response, exhibiting a decrease that, while discernible, did not attain statistical significance in comparison to the control group.