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VOL. 5, ISSUE 4 (2023)
Effects the plants on quorum sensing and biofilm by Acinetobacter baumanii and Pseudomonas aeruginosa
Authors
Salih Mohammed Abdullah, Khansa Mohammed Younis
Abstract
In this study, used the ainetobacter and pseudomonas is called
nosocomial infection and multidrug-resistant and causes many disease such as
bacteremia, pneumonia, meningitis, urinary tract infections and wound
infections. This study aimed to evaluate the effects antibiotics at minimum
inhibitory concentration and sub-minimum inhibitory concentration on biofilm
formation. The plant collection from the market except on collection from tree
and worked extract plant and applied on bacteria as inhibition factors for
bacteria, the first step to determine that’s plants have anti-quorum sensing
activity by used over lay assay which is component two layer of nutrient agar
first layer is solid nutrient agar with volume 10ml and second layer is semi
solid with volume 15ml mixing with serratia marscence worked pore with stable
diameter put 100ul from crude extract of plants after 24h appear result to
determinant of which of plant have anti-quorum sensing or not. Worked biofilm
test and mixing extract of plant with bacteria after 24h show results which of
plant can inhibit bacteria or not, add one step to sure that is crude extract
not kill bacteria take from each concentration 100ul and culture on nutrient
agar.
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Pages:1-7
How to cite this article:
Salih Mohammed Abdullah, Khansa Mohammed Younis "Effects the plants on quorum sensing and biofilm by <i>Acinetobacter baumanii</i> and <i>Pseudomonas aeruginosa</i>". International Journal of Biotechnology and Microbiology, Vol 5, Issue 4, 2023, Pages 1-7
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