Staphylococcus aureus and Enterococcus faecalis were isolated as Gram positive causative agent of UTIs from urine samples of type 1 and type 2 diabetic patients. Phenotyping detection of virulence factors β- hemolysin, biofilm and β-Lactamase was done followed by molecular detection of these factors by PCR using the primers hlB, cyl A, esp and blaZ.

β-hemolysin was highest virulence factors it was produced by all Staphylococcus aureus and Enterococcus faecalis isolates which isolated from both type 1 and type 2 diabetic patients separately. All Enterococcus faecalis isolates which isolated from both type 1 and type 2 diabetic patients separately were produced of biofilm, while Staphylococcus aureus isolates which isolated from both type 1 and type 2 diabetic patients were produced of biofilm with a rate of 40% and 57% respectively. All Staphylococcus aureus isolates which isolated from both type 1 and type 2 diabetic patients separately were produced β-Lactamase, while Enterococcus faecalis isolates which isolated from both type 1 and type 2 diabetic patients were not produced of β-Lactamase.

The hlb gene was appeared in all Staphylococcus aureus which isolated from both type 1 and type 2 diabetic patients separately. The cyl A and esp genes were appeared in all Enterococcus faecalis which isolated from both type 1 and type 2 diabetic patients separately. The lowest appearance of genes was for blaZ gene which was in 60% of Staphylococcus aureus isolated from type 1 diabetic patients, and in 71.4% of isolates which isolated from patients with type 2 diabetes.