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VOL. 5, ISSUE 3 (2023)
The antibacterial effect of Anethum graveolens (dill) on Bacteroides fragilis isolated from clinical samples in Mosul city, Iraq
Authors
Assala Saad Mohsin, Aws Ibrahim Sulaiman
Abstract
Bacteroides fragilis
is a Gram-negative, non-motile, non-spore-forming, and obligate anaerobic
bacterium commonly found in the human colon and other body sites. the leuB
specific gene, known as β-isopropyl malate dehydrogenase, was utilized as a
target for PCR amplification to detect Bacteroides fragilis spp. The leuB
gene plays a crucial role involved in the leucine biosynthesis pathway and
catalyzed the conversion of β-isopropyl malate to α-Ketoiocaproate. Sialidase,
also known as neuraminidase, is a group of enzymes that cleave sialic acid, a
carbohydrate found on the surface of mammalian and microorganism cells. The
nanH gene encodes this enzyme. β-isopropyl malate dehydrogenase found with 100%
sequence identity but lacked the sialidase enzyme gene (nanH). we investigated
the antibacterial activity of Anethum graveolens on B. fragilis
growth. The bacterium displayed high sensitivity to concentrations of 100
μg/ml, resulting in inhibition zones of 25mm and 24mm for the two isolates,
respectively. Decreasing concentrations of 50 μg/ml (15mm, 19mm), 25 μg/ml
(14mm, 14mm), and 12 μg/ml (4mm, 9mm) also exhibited sensitivity.
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Pages:24-27
How to cite this article:
Assala Saad Mohsin, Aws Ibrahim Sulaiman "The antibacterial effect of <i>Anethum graveolens</i> (dill) on <i>Bacteroides fragilis</i> isolated from clinical samples in Mosul city, Iraq". International Journal of Biotechnology and Microbiology, Vol 5, Issue 3, 2023, Pages 24-27
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