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VOL. 5, ISSUE 3 (2023)
Detection of fimv gene in Stenotrophomonas maltophilia isolated from clinical samples
Authors
Bassima Mohammed Ali, Essra Ghanim Al Sammak
Abstract
Stenotrophomonas
maltophilia is a low-virulence opportunistic pathogen that has been identified as
an emerging hospital pathogen. Therefore this study was conducted to
characterize and investigate some virulence factors particularly the detection
of biofilm formation to determine the role of this bacteria in causing
infection in Mosul city. 375 clinical samples were collected from patients
suffering from various infections who frequently visited Mosul hospitals for
both genders. The samples were cultured on MacConkey medium and then incubated
at 37 C°. The results showed that 11 of the isolates were Stenotrophomonas
maltophilia were obtained from different infections, (5)45% of
S. maltophilia was isolated from UTI, (2)18% from
burns, (2)18%from sputum, in addition to (1)9%
from each of wound and medical devices. The bacteria were diagnosed by traditional
methods with the Vitek-2 system in addition to molecular methods based on 16S
rRNA. A slime layer of eleven strains were detected using Congo agar medium.
The results showed that (6)55% of strains gives positive results. while (5)45%
gaves negative results. Biofilm formation by microtiter plate, (8)73% of the strains
were strong and (3)27% were moderate. While the tube method results were
heterogeneous as it showed (5)45% are highly productive, (4)36% are medium, (1)9%
are weak and (1)9% showing no productivity compared to the control. The
adhesion ability was also molecularly diagnosed by designing primers fimv
gene in current study. The results showed that 73%of the strains produced a
positive result after amplifying by PCR at 736 bp.
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Pages:17-23
How to cite this article:
Bassima Mohammed Ali, Essra Ghanim Al Sammak "Detection of fimv <i>gene</i> in <i>Stenotrophomonas maltophilia</i> isolated from clinical samples". International Journal of Biotechnology and Microbiology, Vol 5, Issue 3, 2023, Pages 17-23
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