ARCHIVES
VOL. 5, ISSUE 2 (2023)
The impact of genome editing to improve abiotic stress tolerance in crop
Authors
Riya V Donga, Rajesh K Jha, Mahesh K Saini
Abstract
The availability of genome sequences for
various crops, as well as developments in genome editing techniques, have made
it possible to breed for practically any desirable characteristic. Advances in
genome editing tools such as zinc finger nucleases (ZFNs) and transcription
activator-like effector nucleases (TALENs) have allowed molecular scientists to
target any gene of interest more precisely. CRISPR/Cas9 genome editing, in
contrast to first-generation genome editing techniques, incorporates
straightforward designing and cloning techniques, with the same Cas9
potentially available for usage with different guide RNAs targeting various
locations in the genome. Furthermore, the availability of Cas9 enzymes from
other bacterial species has increased the specificity and efficiency of gene
editing techniques. This review summarises the possibilities useful to plant
biotechnologists for crop improvement utilizing CRISPR/Cas9-based genome
editing technologies, as well as papers demonstrating how CRISPR/Cas9 has been
utilized to improve biotic and abiotic stress tolerance. The primary elements
that affect plant growth and reduce agricultural productivity are biotic
stress like disease and insect pests as well as abiotic stress like
cold, drought, and saline-alkali stress. The application of genome editing
techniques in creating resistant variants has huge potential. These methods
have produced outstanding results in the resistance breeding of significant
vegetable, fruit, and cereal crops like maize, rice, wheat, etc.
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Pages:34-40
How to cite this article:
Riya V Donga, Rajesh K Jha, Mahesh K Saini "The impact of genome editing to improve abiotic stress tolerance in crop". International Journal of Biotechnology and Microbiology, Vol 5, Issue 2, 2023, Pages 34-40
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