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VOL. 5, ISSUE 2 (2023)
Detection of Chlamydia trachomatis in ectopic pregnant women using 16S rDNA genes in Mosul / Iraq
Authors
Hassan A A Al Obeidi, Essra Ghanim A l sammak
Abstract
Three hundred forty (340) swabs were collected
from the cervix to investigate the infection with Chlamydia trachomatis,
of (158women had cervicitis, 95 infertile, 75 were aborted,and12 had an ectopic
pregnancy), by the Obstetrics and gynecologist specialized in Mosul Hospital
and Al-Batool Hospital, Mosul, Iraq, during the period (from Augest 2022 to
Fabruary 2023). Their ages were ranged from (<15-45) years. Three swabs were
taken from the top of the dry vagina, High Vaginal Swabs (H.V.S), with special
dacron swabs, in a circular motion and in one direct direction, with scraping
of the epithelial cells: The 1st.swab for staining with the Giemsa
stain, the 2nd.for the immunological examination of the antigen, and
the 3rd.for the molecular diagnosis by PCR technique to investigate
the gene of 16S rDNA. The results showed that(128),with a rate of (37.6%),were
suffering from clinical symptoms such as vaginal discharge, bleeding, and lower
abdominal pain, and(212)were at a rate of(62.3%).The highest incidence of Chlamydia
trachomatis was in the age group(24-34) years, at a rate of(63.0%),
followed by the age group(15-24)years at a rate of (25.9%), and the lowest
percentage was in the age group(35-44)years at a rate of (11.1%). Dry smears
when stained with giemsa stain were showed the presence of it a ratio of (7.9)340/27.Immunoprecipitation
gave a rate of 340/21(6.1%). Detection of 16S rDNA genes using PCR technique
was indicated infection with it at rate of 340/11(3.2), Most of women ender
study live in city at a percentage of 310(91.1%). The percentage of Country
side women were at (8.9%), and now positive results were recorded for them.
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Pages:21-24
How to cite this article:
Hassan A A Al Obeidi, Essra Ghanim A l sammak "Detection<i> </i>of<i> Chlamydia</i> <i>trachomatis</i> in ectopic pregnant women using 16S rDNA genes in Mosul / Iraq". International Journal of Biotechnology and Microbiology, Vol 5, Issue 2, 2023, Pages 21-24
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