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VOL. 5, ISSUE 2 (2023)
Isolation, identification and antibiotic resistance pattern of Pseudomonas species isolated from poultry feaces in owo metropolis
Authors
Adeluwoye-Ajayi Olayemi Amos, Ajayi Ayorinde, Awoniyi R R
Abstract
Pseudomonas species
are most available pathogenic Gram negative bacteria in environmental samples.
They are resistant to several drugs, opportunistic pathogen and their presence
in the environment poses threat to the public health. The ability of
microorganisms to survive in the presence of antimicrobial agents at a
situation that can kill or enhance their growth is known as antimicrobial
resistance. This has become a serious threat globally, which makes the treatment
of infections in humans and animals expensive and difficult. This study aims at isolating, identifying and investigating the antibiotic resistance pattern of Pseudomonas species isolated from poultry feaces in Owo metropolis. Fresh
feaces of 6 healthy boilers were collected separately into properly labeled and
sterile capped bottles from two different privately owned poultry farms in Owo,
Ondo state Nigeria. Pseudomonas species was isolated using Centrimide
Agar, therefore, their morphological and cultural characteristics on MacConkey
Agar, Nutrient Agar, and Eosin Methylene Blue Agar were equally observed, and
the isolates were therefore characterized. Kirby Bauer disc (KBD) diffusion
method was used for antibiogram of the isolates. The isolates presented
different morphological and cultural characteristics on the saveral types of
media used. At poultry farm A (43.3%) the total prevalence of the Pseudomonas
species in the samples was low compared with (56.7%) at poultry farm B. The
isolates indicated different resistant level to the antibiotics tested with
resistance (3.3%) to the carbapenem (imipenem) and one of the tested cephem
(cefotaxime) being the lowest and resistance (70.0%) to the nitrofuran (nitrofurantoin)
being the highest; while 66.7% were multidrug resistant. This necessitates the
implementation of mitigating strategies to limit the transfer of
antibiotic-resistant Pseudomonas species from animals to humans.
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Pages:16-20
How to cite this article:
Adeluwoye-Ajayi Olayemi Amos, Ajayi Ayorinde, Awoniyi R R "Isolation, identification and antibiotic resistance pattern of <i>Pseudomonas </i>species<i> </i>isolated from poultry feaces in owo metropolis". International Journal of Biotechnology and Microbiology, Vol 5, Issue 2, 2023, Pages 16-20
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