In the present study, the purification of Steviol glycosides from the leaves of S. rebaudiana was carried out using different physical separation techniques such as extraction and crystallization followed by vacuum drying. The extraction of the Steviol glycosides from the leaves of S. rebaudiana was carried out using hot water. The effect of Steviol glycosides to water ratio on extraction efficiency was studied. It was found that the Steviol glycosides to water ratio of 1:35 gave higher percentage recovery of Steviol glycosides. The extract clarification is an important stage as it imparts better visual quality to the final product and this was done using decolouring and flocculating agent such as calcium hydroxide followed by the bleaching treatment using bleaching gas. Subsequent to extract clarification, the techniques of evaporation and crystallization were effectively employed for further purification of Steviol glycosides with a recovery of 95% Steviol glycosides of high purity and reasonably high percentage of rebaudioside A in the product. The process of crystallization was studied in terms of supersaturation (sweeter component). In order to get steviol glycosides crystals in the dry form, vacuum drying was carried out and it was found that steviol glycosides gives crystals in the range 30-70 µm after drying. A new method for HPLC analysis was also developed for the analysis of Steviol glycosides.