Persistent Hepatitis C (HCV) infections may develop liver cirrhosis, which is one of the main causes of liver disease. This study was carried out to determine the seroprevalence of Hepatitis C Virus among blood donors in General Hospital Minna Nigeria. Two hundred (200) samples were collected from consented blood donors in the study area and were examined for anti-HCV antibody using a third-generation enzyme-linked immunosorbent assay (ELISA) Test kit (Auto Bio-Diagnostics, China) based on the manufacturer’s instructions. Out of 200 samples of voluntary blood donors examined, 2.0% were positive for anti-HCV antibody. Female participants had a higher prevalence of 5.3% than Males who had 2.6%. The age group 20-29 years had the highest seroprevalence of 2.6%, followed by age groups 30-39 with a prevalence of 1.8% while age group 10-19, 40-49 and 50years and above showed no evidence of HCV antibodies. The seroprevalence of HCV infections among potential blood donors is clear, and this calls for healthcare professionals and policymakers to ensure that proper screening for HCV is conducted in all healthcare facilities in the area prior to any blood transfusion in order to reduce the spread of the infection in the area and across Nigeria.