Undetected intestinal parasites by routine fecal examination may act as source for transmission of infection through food and drinks. Different techniques are used for detection of intestinal protozoa including direct microscopic examination, concentration techniques and fixed stained smear, however, Microscopical examination is the basic method for routine diagnosis of parasitic infection in stool specimens.71 stool samples were collected from food handlers attending to the Public Health Laboratory, and Bashair hospital, Khartoum, Sudan. All specimens were examined microscopically for parasites using wet mount preparation, formal ether concentration technique, modified ZN-stain, and Gomori’s trichrome stain. (39.4%) of food handlers were harboring commensals, or pathogens. The parasites detected were as follows: Giardia lamblia 6 (8.5%), Entamoeba Histolytica 4 (5.7%), Entamoeba Coli 14 (19.7%), Iodamoeba butcshlii 1 (1.4%), Hymenolepis nana 3 (4.2%) and Cryptosporidium Parvum 1(1.4%). The total number of parasites detected by trichrome stain was 22, by formal ether concentration technique were 21, by wet mount preparation were 18, and by modified ZN-stain was 1 parasite. Trichrome stain is more sensitive and specific than the other methods and is recommended to be added to the diagnostic procedures in the diagnosis of intestinal parasitic diseases.