The aim of this study was to examine the antibacterial activity of turmeric, turmeric plus olive oil and turmeric plus Vaseline against Gram-positive and negative Bacteria. Turmeric, olive oil and Vaseline were purchased from the local market in Riyadh Saudi Arabia. The antibacterial activity of turmeric, turmeric plus olive oil and turmeric plus Vaseline were determined by the agar diffusion method. The tube dilution method was done to determine the minimum inhibitory concentration of the turmeric. The results of the antibacterial activity of turmeric, turmeric plus olive oil and turmeric plus Vaseline against Staphylococcus aureus. Both inhibited the growth of Staphylococcus aureus. Turmeric plus olive oil showed the highest [30mm] whereas turmeric was [10mm] antibacterial activity. When inhibited the growth of E.coli. turmeric plus olive oil showed the highest [18mm] whereas turmeric was [6mm] antibacterial activity. The minimum inhibitory concentration [MIC] of turmeric against microorganisms. The MIC of turmeric ranged from [0.8-1.6 mg/ml]. The lower MIC is an indication of the high effectiveness of turmeric. The blend could generate a new concept for overcoming antibiotic resistance.