The goal of the current study was investigation phytochemical and efficiency of the aqueous, ethanol, and ethyl acetate extracts of Garcinia Cola fruits for antibacterial effects on the bacteria E. coli, Staphylococcus aureus, and Streptococcus mutans. Garcinia Cola fruit was purchased in a market in Macao City in 2022. Preliminary phytochemical screening of the aqueous extracts of Garcinia kola was done to identify the chemical components. Aqueous ethanol, and ethyl acetate extracts were prepared in and used in a pure culture of E. coli, Staphylococcus aureus, and Streptococcus mutans. Paper Standard Disc Diffusion Assay was used to test the antibacterial activity of aqueous ethanol, and ethyl acetate extracts. Phytochemical analysis of the aqueous, Garcinia kola found that the presence of high alkaloids, saponins, flavonoids, tannins, and low cardiac glycosides, cardenolides, steroidal ring, and phlorotannins, Sugar, phlobatanins. The efficacy of water, ethanol, and ethyl acetate extracts of Garcinia Cola fruits against E. coli, Staphylococcus aureus, and Streptococcus mutans was compared, ethanol demonstrated a high inhibition zone in all tested organisms at both concentrations of 250 l/mg and 500 l/mg, followed by ethyl acetate, and finally water extract. The results of this investigation showed that Garcinia Cola fruit was potent against test organism.