A total of twenty five isolates of Aspergillus Niger obtained from soils of Aurangabad region were tested for their efficiency of citric acid production. The isolate number 16 showed maximum citric acid production. This isolate was selected for further study. It was found that the surface fermentation method was more efficient for citric acid production than submerged fermentation on the basis of sucrose consumption. The percentage yield of citric acid on the basis of sugar consumption was higher by surface culture method. The results of titratable acidity also supports the above findings. Citric acid produced was purified and further identified by chromatographic technique.