Plastic are defined as the polymers which are man-made long chain polymer units. There are various methods of polythene degrading but ecofriendly acceptable method is by using microbes. The present study deal with isolation, characterization, screening and identification and enhance degrading activity of bacteria by strain improvement. The different bacteria isolated from different soil (Dumping, Petrol, Village, Village Dump) out of them some bacteria show capacity to use polythene as carbon source and degrade them. The capacity was checked by zone of clearance and growing them on minimal media contain polythene powder as carbon source then the capacity was increased by chemical mutation treatment then observed that the bacteria show more efficiency toward polythene degradation.